In vivo X-ray angiography in the mouse brain using synchrotron radiation.

نویسندگان

  • Keiji Kidoguchi
  • Masahiro Tamaki
  • Takashi Mizobe
  • Junji Koyama
  • Takeshi Kondoh
  • Eiji Kohmura
  • Takashi Sakurai
  • Koichi Yokono
  • Keiji Umetani
چکیده

BACKGROUND AND PURPOSE We, for the first time, performed in vivo x-ray angiography in the mouse brain using SPring-8, a third-generation synchrotron radiation facility. METHODS A thin PE-50 tube was placed in the unilateral external carotid artery in adult male C57BL/6J mice. While maintaining the blood flow in the internal carotid artery, 33 muL of contrast agent was injected and then selective angiography of the hemisphere was performed. RESULTS The average diameters of cerebral artery were as follows: 142.5+/-7.90 microm in middle cerebral artery, 138.3+/-9.35 microm in anterior cerebral artery, 120.5+/-5.53 microm in posterior cerebral artery, and 162.6+/-10.87 microm in internal carotid artery (n=5). To demonstrate the changes in diameter, we induced hypercapnia and detected the dilatation of the vessels between 121% and 124% of the original diameters (n=5). We also repeated angiography in the mice before and after intracarotid injection of vasodilatation drugs papaverine hydrochloride, ATP disodium, and fasudil hydrochloride hydrate and demonstrated the chronological changes in the diameters in each artery at 1, 5, 15, and 30 minutes after injection (n=1 for each drug). CONCLUSIONS Using only a minimum volume of the contrast agent, synchrotron radiation enables us to study x-ray angiography in the mouse brain. The morphology of the vessels can be clearly observed under physiological conditions. The diameters and their changes can also be successfully studied in vivo.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

In Vivo X-ray Angiography of Mouse Brain using Synchrotron Radiation

X-ray angiography using a contrast agent has the main advantage of being able to simultaneously demonstrate the anatomy of vessels and changes in morphology. In rats, morphological changes and different vasoreactivities have been studied by angiography; however, the spatial resolution has not been sufficient to detect detailed changes in vessel diameter. Furthermore, owing to the relatively lar...

متن کامل

A Review of the Applications of Synchrotron Radiation in Archaeological Sciences

The scientific research regarding investigation, characterization and protection of the archeological specimens is manifested through a notable participation of multidisciplinary subjects and experts, scientists and archeometrists. One of the main principals which are considered by archaeometrists in the study of the precious specimens is the utilizing nondestructive methods. As an example, in ...

متن کامل

Detection of breast cancer using non-invasive X-ray diffraction technique of hair: A preliminary study

Background: An early diagnosis of breast cancer relates directly to an accurate treatment plan and strategy. Early detection of breast cancer before its development would be a significant reduction of morbidity and mortality rates. The aim of this preliminary study is to investigate the sensitivity of Wide Angle X-ray diffraction (WAXRD) method on women hair samples of healthy and breast cancer...

متن کامل

Megavoltage X-ray Dose Enhancement with Gold Nanoparticles in Tumor Bearing Mice

One of the applications of gold nanoparticles (GNPs) in medicine is radiation dose-enhancing effect. Although there are many simulations, in vitro and in vivo evidence that GNPs can enhance significantly the radiation dose effect of orthovoltage beams. These beams compared with megavoltage (MV) beams, have limited applications in radiotherapy. In order to evaluate GNPs radiosensitization perfor...

متن کامل

بررسی میزان پرتو پراکنده Xو دوز دریافتی کارکنان بخش آنژیوگرافی یک بیمارستان نظامی

Background and aims: Using X-ray for diagnostic test, is one of the most important exposure to radiation sources for general population and in particular for radiation workers. Therefore, holding down the pollution at the lowest possible level is very important for protection against the internal exposure. The objective of this study is to survey the scattered X-ray rate and received dose by st...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Stroke

دوره 37 7  شماره 

صفحات  -

تاریخ انتشار 2006